Transmission and Scanning Electron Microscopy [ANAT 3003]
Learning Goals & Outcomes
LEARNING GOALS:
This subject will provide training in the theory and practice of operating transmission and scanning electron microscopes, processing biological samples for electron microscopy, ultrathin sectioning, digital imaging, immunological and other techniques required in modern research and hospital electron microscope laboratories. Students will also receive theoretical and practical training in laser scanning confocal microscopy including the use of fluorescent probes to visualise cell organelles and cellular processes.
LEARNING OUTCOMES:
Students who have successfully completed this unit will:
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Have a hands-on knowledge of the operation of transmission and scanning electron microscopes and laser scanning confocal microscopes and a sound understanding of their construction.
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Be able to trouble-shoot problems in operating the microscopes.
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Be able to process, stain and record electron micrographs of biological samples which the student has prepared.
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Be able to identify and correct a tissue preparation problem.
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Be able to interpret biological electron micrographs. Be able to apply their knowledge to completing a project on electron microscopy of a biological sample.
Students will be involved in a variety of learning situations designed to train them for future research and occupational requirements, including: note taking in lectures and tutorials, completion of exercises in the practical book, completion of a project in electron microscopy with Powerpoint© presentation to the group, critique of presentations by other students. In addition to attendance at formal sessions, students will need to take an active role in the learning process through extra reading, extra research based on published articles and websites, practical class activities and extra research for presentation of their own project.
Authors: Anne Swan & Alan Jones, 2002 | Back to top | Course Coordinators